A Guide to Finding the Right Biomarker to Detect in Your Application
Oxidative stress can be evaluated directly by measuring reactive oxygen species (ROS) or indirectly by the associated damage to lipids, proteins, and nucleic acids that occurs upon overproduction of ROS.
Although direct measurement of ROS is ideal, the indirect methods are often relied on more heavily due to the relative stability of damage markers on biomolecules compared to the transient nature of ROS. To help you determine which is best to use in your experimental system, here is a breakdown of the assay technology used to detect the most common oxidative damage biomarkers.
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